Mango seedlings as affected by fungus Colletotrichum gloeosporioides through different inoculation methods

Rawal Ahmed Qambrani, Muhammad Ibrahim Khaskheli, Sajad Ali Khaskheli and Zareen Qambrani

ABSTRACT    This study aimed to evaluate mango seedlings as affected by fungus Colletotrichum gloeosporioides through different inoculation methods. Pathogenicity test was conducted under controlled conditions by using different inoculation methods for confirmation of pathogen, i.e., spraying after rupturing method (M1), spraying method (M2), Injecting method (M3) and Disk method (M4). The data was recorded as plant height, infected number of leaves, numbers and size of lesions after every 15 days of interval 15, 30 and 45 days after inoculation. The maximum disease incidence was recorded in (M1) followed by (M2), (M3) and (M4). Minimum plant height was recorded in (M1) followed by (M2), (M3) and (M4). The maximum number of necrotic spots on plants were recorded in (M1) followed by (M2), (M3) and (M4), whereas no necrotic lesions were recorded in control. Initially, in 15 days minimum necrotic lesions were observed, while maximum necrotic lesions were recorded in 30 and 45 days. The necrotic spots per plant were recorded in (M1) followed by (M2), (M3) and (M4). However, the maximum necrotic spots length for different days was recorded in 45 followed by 30 and 15 days. Whereas, the interaction between methods and days represents the maximum length was recorded 45 days in (M1). The interaction between days after inoculation and methods represents that maximum width of necrotic spots were recorded 45 days in (M1). The total size of necrotic spots (length + width) was recorded in (M1) followed by (M2), (M3) and (M4). The maximum size was recorded in 45 days. The interaction with total (length + width) size of necrotic spots for days and methods represents that the maximum size was recorded 45 days in (M1). © 2022 Department of Agricultural Sciences, AIOU

Keywords: Anthracnose, Colletotrichum gloeosporioides, Inoculation methods, Mango, Seedlings, Pathogenicity

Permanent link to this article: https://jpaa.aiou.edu.pk/?p=1255